814702

TOYOPEARL^® Butyl-650M Bulk Media

phase C4 (butyl), bottle of 1 L, 65 μm particle size

• Sinônimo(s): TOYOPEARL^® Butyl-650 HIC Bulk Media
• Código UNSPSC: 41115700

Propriedades

• descrição: Butyl 650M
• linha de produto: TOYOPEARL^®
• Formulário: slurry
• embalagem: bottle of 1 L
• fabricante/nome comercial: Tosoh 14702
• Parâmetros: 3 bar max. pressure
• técnica(s): LPLC: suitable
• Matriz: hydroxylated methacrylic polymer
• Grupo ativo da matriz: C4 (butyl) phase
• tamanho de partícula: 65 μm
• pH operacional: 1-13
• técnica de separação: hydrophobic interaction (HIC)

Descrição

Descrição geral

TOYOPEARL^® HIC resins are hydrophobic interaction chromatography resins that offer the following advantages: strong affinity for water-soluble proteins, high recovery of mass and activity, high sample capacity up to 2-4 times that of gel media, fluctuating salt concentrations will not change the bed volume, mechanical stability to 7kg/cm squared (7 bar/100psi), stability at a wide pH range (2-12), clean in place with 0.5M NaOH, and autoclavable. The exclusion limit of 5x10⁶ Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects.

TOYOPEARL^® HIC resins for hydrophobic interaction chromatography offer these advantages:

• Strong affinity for water-soluble proteins
• High recovery of mass and activity
• High sample capacity: up to 2-4 times that of gel media
• Fluctuating salt concentrations will not change bed volume
• Mechanical stability to 7kg/cm² (7 bar/100psi)
• Stable at wide pH range: 2-12
• Clean in place with 0.5M NaOH
• Autoclavable

     
TOYOPEARL media offer high yield recovery of proteins, using various aqueous eluants. The exclusion limit of 5x10⁶ Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects. Packed as 100mL bottle.

Especificações

Clean in place with 0.5 M NaOH or 0.1 M HCl.

forma física

72% (v/v) slurry in 20% (v/v) ethanol

Shipped in 20% (v/v) ethanol.

Outras notas

Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in interresinte purification and polishing steps

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Informações legais

Toyopearl is a registered trademark of Tosoh Corporation

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Danger
• Frases de perigo: H225
• Declarações de precaução: P210 - P233 - P240 - P241 - P242 - P243
• Classificações de perigo: Flam. Liq. 2
• Código de classe de armazenamento: 3 - Flammable liquids
• Classe de risco de água (WGK): WGK 3