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T3038

Sigma-Aldrich

Trizma® hydrochloride solution

1 M, BioReagent, for molecular biology

Sinônimo(s):

Tris hydrochloride solution

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About This Item

Número MDL:
MFCD00012590
Código UNSPSC:
12161700
ID de substância PubChem:
24900102
NACRES:
NA.25

grau

for molecular biology
for molecular biology

Nível de qualidade

200

esterilidade

0.2 μm filtered

linha de produto

BioReagent

forma

solution

concentração

1 M

Impurezas

DNase, RNase, NICKase and protease, none detected

pH

8.0

aplicação(ões)

agriculture

cadeia de caracteres SMILES

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

chave InChI

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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Descrição geral

Trizma® hydrochloride solution is a useful biological buffer.

Aplicação

Trizma® hydrochloride solution may be used in the following studies:
  • As buffer for the 2-D electrophoresis of rat fibroblast cell.
  • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
  • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Outras notas

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Informações legais

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Lisa W von Friesen et al.
Marine pollution bulletin, 142, 129-134 (2019-06-25)
Standardized methods for the digestion of biota for microplastic analysis are currently lacking. Chemical methods can be effective, but can also cause damage to some polymers. Enzymatic methods are known to be gentler, but often laborious, expensive and time consuming.
P R Langer et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(11), 6633-6637 (1981-11-01)
Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and
M G Murray et al.
Nucleic acids research, 8(19), 4321-4325 (1980-10-10)
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA
Emilie M F Kallenbach et al.
The Science of the total environment, 786, 147455-147455 (2021-05-09)
Chitinaceous organisms have been found to ingest microplastic; however, a standardised, validated, and time- and cost-efficient method for dissolving these organisms without affecting microplastic particles is still required. This study tested four protocols for dissolving organisms with a chitin exoskeleton:
F Gharahdaghi et al.
Electrophoresis, 20(3), 601-605 (1999-04-27)
Mass spectrometry is a powerful technique for the identification of proteins at nanogram quantities. However, some degree of sample preparation prior to mass spectrometry is required, and silver-stained protein gel samples are most problematic. Here we report our strategy to
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