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Key Documents

T2194

Sigma-Aldrich

Trizma® hydrochloride solution

pH 7.4, BioPerformance Certified, 1 M, suitable for cell culture

Sinônimo(s):

Tris hydrochloride solution

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About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

grau

BioPerformance Certified
for molecular biology

Nível de qualidade

200

forma

solution

concentração

1 M

técnica(s)

cell culture | mammalian: suitable

Impurezas

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.4

faixa de pH útil

7.0-9.0

absorção

≤0.05 at 290 at 40%

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Descrição geral

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Aplicação

Trizma®hydrochloride solution has been used:
  • in the preparation of cell-lysis buffer
  • as a component of wash buffers and RNase mix 1, used in the preparation of 5′-complete cDNAs for paired-end sequencing
  • in dissolving cadmium or S2- loaded microreactors for CdS synthesis

Informações legais

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Determination of Rate of [3H-methyl]-choline Incorporation into Cellular Lipids and Non-lipid Metabolites
Smith T and Phyu S
Bio-protocol, 6(22) (2016)
RAMPAGE: Promoter Activity Profiling by Paired-End Sequencing of 5?-Complete cDNAs
Batut P and Gingeras TR
Current Protocols in Molecular Biology, 104(1), 25B-211 (2013)
Lab-on-a-micromotor: catalytic Janus particles as mobile microreactors for tailored synthesis of nanoparticles
Pacheco M, et al.
Chemical Science, 9(42), 8056-8064 (2018)
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
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Nature communications, 11(1), 6299-6299 (2020-12-09)
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