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Merck
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Documentos Principais

T2036

Sigma-Aldrich

apo-Transferrina

powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

Sinônimo(s):

Siderofilina, Transferrina humana

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About This Item

Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.75

linha de produto

BioReagent

Ensaio

≥98% (agarose gel electrophoresis)

Formulário

powder

peso molecular

76-81 kDa

concentração

~25 mM

técnica(s)

cell culture | mammalian: suitable

Impurezas

HIV and HBsAg, source material tested negative
endotoxin, tested

solubilidade

H2O: 50 mg/mL

traços de cátion

Fe: ≤0.005%

nº de adesão UniProt

Condições de expedição

ambient

temperatura de armazenamento

−20°C

Informações sobre genes

human ... TF(7018)

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Descrição geral

Transferrin, majorly synthesized in the liver, is an abundant glycoprotein in the serum. The members of the transferrin superfamily have similar polypeptide folding patterns. Transferrin contains N- and C-terminal iron-binding homologous domains. Each of these domains is split into two subdomains having binding sites for iron and anions within the inter-subdomain cleft. The binding cleft opens and closes with iron releasing and iron binding. apo-Transferrin is an iron-free protein that arises after transferrin dissociates from its receptor. This product can be supplemented with iron or used to bind free iron present in media.

Aplicação

apo-Transferrin human has been used:
  • to culture human primary pancreatic endothelial cells, Het1As (non-tumorous esophagus cells), and immortalized human colonic epithelial cells (HCEC-1CT)
  • to culture α mouse liver 12 (AML-12) (mature hepatocytes) cells
  • as a source for human apo-transferrin for purification before crystallography

Ações bioquímicas/fisiológicas

Transferrin is responsible for transporting iron from the sites of absorption and storage to the tissue cells. It maintains the levels of iron in biological fluids. The levels of transferrin may indicate the total iron-binding capacity (TIBC). Transferrin supplies the required iron for incorporation into hemoglobin within RBCs in the bone marrow. Iron deficiency causes an increase in the levels of transferrin. Pregnancy and estrogen administration can also raise the levels of transferrin. Chronic liver disease, renal insufficiency, malnutrition, and protein-losing enteropathies reduce the synthesis of transferrin.

Outras notas

Proteína transportadora de ferro não heme.

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3


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