SRP0708

BTK (C481S), Active

PRECISIO^® Kinase, recombinant

• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• recombinante: expressed in baculovirus infected Sf9 cells
• Ensaio: 70-95% (SDS-PAGE)
• forma: frozen liquid
• atividade específica: 40-56 nmol/min·mg (radiometric)
• peso molecular: ~75 kDa
• concentração: 0.1 μg/μL
• nº de adesão: NM_005228
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C

Descrição

Aplicação

Useful for kinase assay and Western blot.

Ações bioquímicas/fisiológicas

Recombinant full-length human BTK (C481S) was expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation. BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors ⁽¹⁾.Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. The unique role of BTK makes it a desirable target for potential anti-cancer, anti-inflammatory and anti-viral agents as well as other treatments ⁽²⁾.

Reconstituição

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

Armazenamento e estabilidade

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Informações legais

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS08,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H315,H319,H360D
• Declarações de precaução: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classificações de perigo: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable