S7897
Sarcosine Oxidase from Bacillus sp.
lyophilized powder, 25-50 units/mg solid
Sinônimo(s):
Sarcosine: oxygen oxidoreductase (demethylating)
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About This Item
Produtos recomendados
Formulário
lyophilized powder
Nível de qualidade
atividade específica
25-50 units/mg solid
peso molecular
44 kDa
temperatura de armazenamento
−20°C
Descrição geral
Monomeric sarcosine oxidase (MSOX) is a flavoenzyme that catalyzes the oxidative demethylation of sarcosine (N-methylglycine) to yield glycine, formaldehyde, and hydrogen peroxide. Monomeric sarcosine oxidase can oxidize other secondary amino acids such as N-methyl-L-alanine, N-ethylglycine, and L-proline.
Sarcosine oxidase is a 44 kDa protein with covalently bound 1 mol of flavin adenine dinucleotide (FAD). It belongs to the family of amino acid oxidases with bound FAD.
Aplicação
Sarcosine oxidase from Bacillus sp. has been used as a positive control for detection of proteins with covalently bound flavin adenine dinucleotide (FAD) by UV. It has also been used in co-immobilization with other enzymes on platinum electrode for preparation of creatinine biosensor.
Sarcosine oxidase has been used in a study as part of a multienzyme cascade, that when immobilized constructed amperometric biosensors. Sarcosine oxidase has also been used in a study to investigate oxidation of amines by flavoproteins.
Definição da unidade
One unit will form 1.0 μmole of formaldehyde from sarcosine per min at pH 8.3 at 37 °C.
forma física
No stabilizers added
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Wolff AM, et al.
Protein Expression and Purification, 22(2), 189-199 (2001)
Handbook of Flavoproteins: Oxidases, Dehydrogenases and Related Systems, 196-196 (2012)
Paul F Fitzpatrick
Archives of biochemistry and biophysics, 493(1), 13-25 (2009-08-05)
Many flavoproteins catalyze the oxidation of primary and secondary amines, with the transfer of a hydride equivalent from a carbon-nitrogen bond to the flavin cofactor. Most of these amine oxidases can be classified into two structural families, the D-amino acid
Rastislav Monošík et al.
Analytical biochemistry, 421(1), 256-261 (2011-11-10)
Amperometric biosensors based on gold planar or nanocomposite electrode containing multiwalled carbon nanotubes for determination of glycerol were developed. The biosensors were constructed by immobilization of a novel multienzyme cascade consisting of glycerol kinase/creatine kinase/creatinase/sarcosine oxidase/peroxidase between a chitosan "sandwich."
Alshaimaa Hassan-Abdallah et al.
Biochemistry, 47(9), 2913-2922 (2008-02-07)
Monomeric sarcosine oxidase (MSOX) contains covalently bound FAD and catalyzes the oxidative demethylation of sarcosine ( N-methylglycine). The side chain of Arg49 is in van der Waals contact with the si face of the flavin ring; sarcosine binds just above
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