S0266

Spermidine

BioReagent, for molecular biology, suitable for cell culture, ≥98%

• Sinônimo(s): 1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane
• Fórmula linear: NH₂(CH₂)₃NH(CH₂)₄NH₂
• Número CAS: 124-20-9
• Peso molecular: 145.25
• Beilstein: 1698591
• Número CE: 204-689-0
• Número MDL: MFCD00008229
• Código UNSPSC: 12352200
• ID de substância PubChem: 24899434
• NACRES: NA.52

Propriedades

• grau: for molecular biology
• Nível de qualidade: 200
• linha de produto: BioReagent
• Ensaio: ≥98%
• forma: solid or liquid
• peso molecular: 145.25 (AMU)
• técnica(s): cell culture | mammalian: suitable
• índice de refração: n20/D 1.479 (lit.)
• densidade: 0.925 g/mL at 25 °C (lit.)
• atividade externa: DNase, RNase, and protease, none detected
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: NCCCCNCCCN
• InChI: 1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
• chave InChI: ATHGHQPFGPMSJY-UHFFFAOYSA-N
• Informações sobre genes: rat ... Ppm1a(24666)

Descrição

Descrição geral

Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.

Aplicação

Spermidine has been used in the preparation of gold beads and as a component in nuclei isolation buffer (NIB).

Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.

Ações bioquímicas/fisiológicas

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T₄ polynucleotide kinase activity.

Spermidine helps to maintain the proper activity of gene expression. This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300). Spermidine participates in cell growth.

Atenção

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Informações de segurança

• Pictogramas: GHS05
• Palavra indicadora: Danger
• Frases de perigo: H314
• Declarações de precaução: P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405
• Classificações de perigo: Eye Dam. 1 - Skin Corr. 1B
• Código de classe de armazenamento: 8A - Combustible, corrosive hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): 233.6 °F - closed cup
• Ponto de fulgor (°C): 112 °C - closed cup
• Equipamento de proteção individual: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter