PLEX15

GenElute^™ Endotoxin-free Plasmid Maxiprep Kit

sufficient for 15 purifications

• Sinônimo(s): GenElute^™ Endotoxin-free Plasmid Kits, Gen Elute
• Código UNSPSC: 41105501
• NACRES: NA.52

Propriedades

• uso: sufficient for 15 purifications
• características do produto alternativo mais ecológico: Inherently Safer Chemistry for Accident Prevention; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• técnica(s): DNA purification: suitable
• categoria alternativa mais ecológica: , Aligned
• temperatura de armazenamento: 15-25°C

Descrição

Descrição geral

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.

Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 μg of endotoxin-free plasmid DNA may be prepared from 5 to 40 mL of culture.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.

Aplicação

The kit has been used to purify plasmid DNA for high efficiency transfection.

Características e benefícios

• High transfection efficiency and yield
• Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
• Fast and simple allowing up to 12 preps in less than 2 hours

Informações legais

GenElute is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Pictogramas: GHS08,GHS05,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H290,H302 + H332,H315,H319,H334,H336
• Declarações de precaução: P234 - P261 - P264 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Central nervous system
• Código de classe de armazenamento: 8A - Combustible corrosive hazardous materials
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable