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Key Documents

P8999

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone DO-7, purified from hybridoma cell culture

Sinônimo(s):

Anti-p53 antibody, Mouse monoclonal, Anti-TRP53, Anti-Transformation-related protein 53

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

DO-7, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~53 kDa

reatividade de espécies

human

concentração

~2 mg/mL

técnica(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using human A431

Isotipo

IgG2b

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... TP53(7157)

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Descrição geral

Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues.

Aplicação

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation

Ações bioquímicas/fisiológicas

Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21waf1. p21waf1 (cyclin-dependent kinase inhibitor 1A) interacts directly with cyclin dependent kinases, important for cell cycle progression, thereby inhibiting their activity and resulting in cell cycle arrest.
p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

DNA damage-induced activation of p53 by the checkpoint kinase Chk2
Hirao A, et al.
Science (New York, N.Y.), 287(5459), 1824-1827 (2000)
New Insights into p53 Signaling and Cancer Cell Response to DNA Damage: Implications for Cancer Therapy.
Mirzayans, R., et al.
Journal of Biomedicine and Biotechnology, doi: 10-doi: 10 (2012)
Role of p53, apoptosis, and cell proliferation in early stage Epstein-Barr virus positive and negative gastric carcinomas
Ishii HH, et al.
Journal of Clinical Pathology, 57(12), 1306-1311 (2004)
Chk2/hCds1 functions as a DNA damage checkpoint in G1 by stabilizing p53
Chehab NH, et al.
Genes & Development, 14(3), 278-288 (2000)
Predictors of progression in Barrett's esophagus II: baseline 17p (p53) loss of heterozygosity identifies a patient subset at increased risk for neoplastic progression
Reid BJ, et al.
The American Journal of Gastroenterology, 96(10), 2839-2839 (2001)

Artigos

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

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