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Key Documents

P7874

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone DO-2, purified from hybridoma cell culture

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

purified from hybridoma cell culture

tipo de produto de anticorpo

primary antibodies

clone

DO-2, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~53 kDa

reatividade de espécies

human

concentração

~2 mg/mL

técnica(s)

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using total cell extract of A431 cells

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... TP53(7157)

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Descrição geral

Monoclonal Anti-p53 (mouse IgG1 isotype) is derived from the hybridoma DO-2 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53.
p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Imunogênio

recombinant human wild type p53. The antibody epitope resides between amino acids 10-16 of human p53.

Aplicação

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for DNase I footprint, immunoprecipitation and western blotting.

Ações bioquímicas/fisiológicas

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.
p53 is phosphorylated at multiple sites by several protein kinases in response to DNA damage. It is essential for the maintenance of the non-tumorigenic phenotype of cells. Phosphorylation of p53 at Ser15 by ataxia telangiectasia mutate (ATM), ataxia telangiectasia and Rad3-related protein (ATR), and DNA-dependent protein kinase (DNA-PK) leads to a reduced interaction with its negative regulator MDM2 and accumulation of p53 protein. Checkpoint kinase 2 (Chk2) and Chk1 can phosphorylate p53 at Ser20, which enhances its activity, tetramerization, and stability. Elevation of p53 protein induces the transcriptional activation of multiple genes, including cyclin-dependent kinase inhibitor 1 (p21waf1).

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

p53 C-terminal phosphorylation by CHK1 and CHK2 participates in the regulation of DNA-damage-induced C-terminal acetylation
Ou YH, et al.
Molecular Biology of the Cell, 16(4), 1684-1695 (2005)
Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
Wild-type p53 protein is up-regulated upon cyclic adenosine monophosphate-induced differentiation of human endometrial stromal cells
Pohnke Y, et al.
The Journal of Clinical Endocrinology and Metabolism, 89(10), 5233-5244 (2004)
Regulation of the Mdm2-p53 signaling axis in the DNA damage response and tumorigenesis
Carr MI and Jones SN
Translational Cancer Research, 5(6), 707-707 (2016)
Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)

Artigos

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

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