P2192
10X PCR Buffer
Optimized for routine PCR with MgCl2 included
Sinônimo(s):
PCR Buffer Solution, PCR Reaction Buffer
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About This Item
Código UNSPSC:
41106306
NACRES:
NA.52
Produtos recomendados
Nível de qualidade
Formulário
liquid
embalagem
vial of 1.5 mL
cor
colorless
aplicação(ões)
agriculture
atividade externa
DNase, RNase, none detected
temperatura de armazenamento
−20°C
Categorias relacionadas
Aplicação
10X PCR Buffer has been used as a component of the PCR mixture:
- for the amplification of viral DNA
- to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
- to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.
Características e benefícios
- Standalone buffer
- Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)
Componentes
Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin
Informações legais
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves
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The purpose of this study was to evaluate the relationship between 5,10- methylenetetrahydrofolate reductase (MTHFR) polymorphisms and Attention Deficit Hyperactivity Disorder (ADHD) in a sample of Turkish children. MTHFR gene polymorphisms were assessed in 40 patients with ADHD and 30
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Protocolos
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
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