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Documentos Principais

DNTP100A

Sigma-Aldrich

Deoxynucleotide Set, 100 mM

Individual dNTPs for routine PCR; 1 mL each

Sinônimo(s):

PCR dNTPs, dNTP set, dNTPs

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About This Item

Código UNSPSC:
41106305
NACRES:
NA.52

Nível de qualidade

forma

liquid

concentração

100 mM

cor

colorless

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

Deoxynucleotide triphosphates (dNTPs) are negatively charged organic molecules, which contains nucleobase bound to deoxyribose. This dNTPs set includes 1.0 mL each of 100mM aqueous solutions of four deoxyribonucleotides namely deoxyribose adenosine triphosphate (dATP), deoxyribose cytidine triphosphate (dCTP), deoxyribose guanosine triphosphate (dGTP), and deoxyribose thymidine triphosphate (dTTP) in separate tubes for convenience and easy PCR reaction setup.

Aplicação

Deoxynucleotide set, 100 mM has been used in:
  • the amplification of DNA in polymerase chain reaction (PCR)
  • quantitative PCR (qPCR)
  • multiplex PCR

Ações bioquímicas/fisiológicas

Deoxynucleotide triphosphates (dNTPs) are building blocks of nucleic acid molecules and are acidic in nature. They act as substrates for DNA polymerizing enzymes. dNTPs are essential components of PCR master mixes as it is necessary for DNA amplification reaction. Optimal levels of dNTPs ensure proper chromosomal replication and in turn maintain genome stability.

Características e benefícios

  • Provided as 4 separate tubes of dNTPs
  • Allows optimization of individual dNTP concentrations

Embalagem

1.0 mL each of 100 mM dATP, dCTP, dGTP and dTTP

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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