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N3254

Sigma-Aldrich

4-Nitrophenyl phosphate di(tris) salt

phosphatase substrate, ≥97% (enzymatic), powder

Sinônimo(s):

4-Nitrophenyl phosphate bis(tris) salt

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About This Item

Fórmula linear:
(C6 H4 N O6 P)( C4 H12 N O3)2
Número CAS:
68189-42-4
Peso molecular:
461.36
Beilstein:
1578142
Número CE:
269-198-6
Número MDL:
MFCD00037009
Código UNSPSC:
12352204
ID de substância PubChem:
24897633
NACRES:
NA.83

product name

4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

Nível de qualidade

100

Ensaio

≥97% (enzymatic)

forma

powder

solubilidade

water: 50 mg/mL, clear, colorless to light yellow

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

chave InChI

XXAXKCWOTRABOW-UHFFFAOYSA-N

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Aplicação

4-Nitrophenyl phosphate di(tris) salt has been used:
  • as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
  • to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
  • for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Ações bioquímicas/fisiológicas

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

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Enzyme-linked Immunosorbent Assay (ELISA): From A to Z null
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Chronic wasting disease (CWD) is a fatal prion disease affecting multiple cervid species. Effective management tools for this disease, particularly in free-ranging populations, are currently limited. We evaluated a novel CWD vaccine in elk (Cervus canadensis) naturally exposed to CWD
Ellen K Wasan et al.
Vaccine, 37(11), 1503-1515 (2019-02-12)
We previously developed an highly efficacious combination adjuvant comprised of innate defense regulator (IDR)-1002 peptide, poly(I:C) and polyphosphazene (TriAdj). Here we aimed to design and test the in vivo efficacy of a mucoadhesive nasal formulation of this adjuvant. To determine
Karol Bomsztyk et al.
Nucleic acids research, 47(12), e69-e69 (2019-03-31)
Chromatin immunoprecipitation (ChIP) is the most widely used approach for identification of genome-associated proteins and their modifications. We have previously introduced a microplate-based ChIP platform, Matrix ChIP, where the entire ChIP procedure is done on the same plate without sample

Artigos

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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