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MIDI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Sinônimo(s):

Green PKH membrane midi labeling kit

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.32

embalagem

pkg of 1 kit

Nível de qualidade

100

fabricante/nome comercial

Distributed for Phanos Technologies

condição de armazenamento

protect from light

técnica(s)

flow cytometry: suitable

fluorescência

λex 490 nm; λem 502 nm (PKH67 dye)

aplicação(ões)

cell analysis
detection

método de detecção

fluorometric

Condições de expedição

ambient

temperatura de armazenamento

room temp

Descrição geral

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Aplicação

PKH67 Green Fluorescent Cell Linker Midi Kit has been used for:
  • labeling exosomes  
  • extracellular vesicles (EVs)l
  • normal and sickle red blood cells (RBCs)
  • Raji cells

Ligação

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Informações legais

CellVue is a registered trademark of Phanos Technologies

Somente componentes do kit

Nº do produto
Descrição
  • PKH67 Dye 2 x 0.1
  • Diluent C 6 x 10

Pictogramas

FlameExclamation mark
GHS02,GHS07

Palavra indicadora

Danger

Frases de perigo

H225,H319

Classificações de perigo

Eye Irrit. 2 - Flam. Liq. 2

Código de classe de armazenamento

3 - Flammable liquids

Ponto de fulgor (°F)

57.2 °F - closed cup

Ponto de fulgor (°C)

14 °C - closed cup

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Dongxin Tang et al.
Clinical and translational medicine, 10(4), e139-e139 (2020-09-09)
Cancer stem cells (CSCs) are important factors contributing to tumorigenesis. We examined whether CSCs isolated from colorectal cancer (CRC) cells possess metastatic properties that can be transferred to non-CSCs via the delivery of miR-200c enclosed in extracellular vesicles (EVs). The
Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells
Bach F, et al.
Testing, 8(51), 88845-88845 (2017)
Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative
Alshaiban A, et al.
Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 22, 230-238 (2016)
ADCC Assay Protocol
Fang L and He F
Journal of Virology (2013)
Chuang Gao et al.
Aging, 11(24), 12147-12164 (2019-12-17)
Exosomes are small (30-150 nm diameter) lipid bilayer-enclosed vesicles found in all bodily fluids. We investigated whether exosomes play a role in chronic subdural hematoma (CSDH). Exosomes were identified and characterized using transmission electron microscopy and NanoSight particle tracking. The
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