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PKH67GL

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Sinônimo(s):

Green PKH membrane labeling kit

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.32

Nível de qualidade

200

embalagem

pkg of 1 kit

condição de armazenamento

protect from light

fluorescência

λex 490 nm; λem 502 nm (PKH67 dye)

método de detecção

fluorometric

Condições de expedição

ambient

temperatura de armazenamento

room temp

Categorias relacionadas

Aplicação

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling has been used:
  • To label and further investigate the exosomes expelled from cells in triple-negative breast cancer condition.
  • To label apoptotic cells, in order to study the role of ανβ5 receptor in both binding and internalization of apoptotic cells.
  • In fluorescence imaging.

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Ligação

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Informações legais

CellVue is a registered trademark of Phanos Technologies

Somente componentes do kit

Nº do produto
Descrição
  • Diluent C 6 x 10
  • PKH67 Cell Linker in ethanol .5 mL

Pictogramas

FlameExclamation mark
GHS02,GHS07

Palavra indicadora

Danger

Frases de perigo

H225,H319

Classificações de perigo

Eye Irrit. 2 - Flam. Liq. 2

Código de classe de armazenamento

3 - Flammable liquids

Ponto de fulgor (°F)

57.2 °F - closed cup

Ponto de fulgor (°C)

14 °C - closed cup

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
MKCP3216
MKCQ0194
MKCK9600
SLBX5620
SLBW9960

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alpha v beta 5 integrin recruits the CrkII-Dock180-Rac1 complex for phagocytosis of apoptotic cells
Albert, Matthew L and Kim, Jong-Ii and Birge, Raymond B
Nature Cell Biology, 2(12), 899-899 (2000)
Increased expression of inducible HSP70 in apoptotic cells is correlated with their efficacy for antitumor vaccine therapy
Masse D, et al.
International Journal of Cancer. Journal International Du Cancer, 111(4), 575-583 (2004)
Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
O Brien K, et al.
European Journal of Cancer, 49(8), 1845-1859 (2013)
Anjali P Kusumbe et al.
Stem cells (Dayton, Ohio), 27(3), 498-508 (2009-03-04)
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited
Z M Wu et al.
Placenta, 33(3), 188-194 (2012-01-04)
As human blastocyst-derived extravillous trophoblasts (EVTs) invade the early decidua, they are positioned to interact with immune cells and resident decidual cells, and remodel spiral arteries into high capacity vessels that increase blood flow to the developing fetal-placental unit. Shallow
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