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MINCLARET

Sigma-Aldrich

CellVue® Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labeling

Distributed for Phanos Technologies

Sinônimo(s):

Far red membrane labeling kit

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.32

embalagem

pkg of 1 kit

Nível de qualidade

fabricante/nome comercial

Distributed for Phanos Technologies

condição de armazenamento

protect from light

técnica(s)

flow cytometry: suitable

fluorescência

λex 655 nm; λem 675 nm (CellVue claret dye)

aplicação(ões)

cell analysis
detection

método de detecção

fluorometric

Condições de expedição

ambient

temperatura de armazenamento

room temp

Aplicação

CellVue Claret kit fluoresces in the far red and may be used for general cell membrane labeling including in vitro cell labeling, in vitro lymphocyte proliferation studies,† in vivo cytotoxicity assays,† and also as a second color for monitoring cell-cell membrane transfer.†
CellVue® Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labeling has been used:
  • to label monocyte-derived macrophages (MDMs) for antibody-dependent cellular phagocytosis assay
  • in labeling of Jurkat cells after the induction of apoptosi
  • in labeling of cells in mice for assaying the retention of tumor cells in lung

Ações bioquímicas/fisiológicas

The CellVue Claret Fluorescent Cell Linker kit uses a proprietary membrane labeling technology to stably incorporate a far red fluorescent dye with long aliphatic tails (CellVue Claret) into lipid regions of the cell membrane.†

Embalagem

This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.

Ligação

For additional technical details on Fluorescent Cell Linker Dyes including a comprehensive bibliography, please visit PKH and CellVue®; Claret Fluorescent Cell Linker Dyes.

Informações legais

Distributed for Phanos Technologies, Inc.
Patent Information
CellVue is a registered trademark of Phanos Technologies

Pictogramas

FlameExclamation mark

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Flam. Liq. 2

Classe de risco de água (WGK)

WGK 1


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Visite a Biblioteca de Documentos

Mitochondrial fission promotes the continued clearance of apoptotic cells by macrophages
Wang Y, et al.
Cell, 171(2), 331-345 (2017)
Comparative assessment of clinically utilized CD20-directed antibodies in chronic lymphocytic leukemia cells reveals divergent NK cell, monocyte, and macrophage properties
Rafiq S, et al.
Journal of immunology (Baltimore, Md. : 1950), 190(6), 2702-2711 (2013)
The detergent-soluble cytoplasmic pool of survivin suppresses anoikis and its expression is associated with metastatic disease of human colon cancer
Hori M, et al.
Testing, 8(2), e55710-e55710 (2013)
Jingting Li et al.
Biomaterials, 35(2), 642-653 (2013-10-24)
Autologous cells suffer from limited cell number and senescence during ex vivo expansion for cartilage repair. Here we found that expansion on extracellular matrix (ECM) deposited by fetal synovium-derived stem cells (SDSCs) (FE) was superior to ECM deposited by adult
Sudjit Luanpitpong et al.
Environmental science & technology, 48(21), 12912-12919 (2014-10-28)
Epidemiological studies suggest that living near mountaintop coal mining (MTM) activities is one of the contributing factors for high lung cancer incidence. The purpose of this study was to investigate the long-term carcinogenic potential of MTM particulate matter (PMMTM) exposure

Artigos

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

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