MAK524

Isocitrate Assay Kit

sufficient for 100 colorimetric tests

• Código UNSPSC: 12161503
• NACRES: NA.84

Propriedades

• Nível de qualidade: 100
• aplicação(ões): food and beverages
• método de detecção: colorimetric
• doença(s) relevante(s): cancer
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Isocitrate (isocitric acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.

Características e benefícios

• Linear detection range: 20 to 5000 µM
• Compatible with high-throughput handling systems.

Adequação

The kit is suitable for isocitrate determination in food, beverages, and biological samples (e.g. cell lysate, tissue homogenate, serum, etc.).

Princípio

The Isocitrate Assay Kit measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2