MAK517

Glutathione (GSH) Assay Kit

sufficient for 250 colorimetric tests

• Código UNSPSC: 12161503
• NACRES: NA.84

Propriedades

• Nível de qualidade: 100
• aplicação(ões): pharmaceutical
• método de detecção: colorimetric
• doença(s) relevante(s): cancer
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Glutathione is a tripeptide of glycine, glutamic acid and cysteine. In the red blood cell, the reduced form of glutathione is vital in maintaining hemoglobin in a reduced state and hence protecting the cells from oxidative damage. Glutathione is involved in detoxification of hydrogen peroxide through glutathione oxidase. Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6-phosphate dehydrogenase, glutathione synthase and glutathione reductase.

Características e benefícios

• Linear detection range: 0.4 - 100 µM
• No interference from amino acids and common buffers.

Adequação

The kit is suitable for the determination of reduced glutathione in whole blood, plasma, serum, urine, tissue, and cell extracts, as well as for studying the effects of drugs on glutathione metabolism.

Princípio

The Glutathione (GSH) Assay Kit is designed to accurately measure reduced glutathione in biological samples. The improved 5,5′-dithiobis(2-nitrobenzoic acid (DTNB) method combines deproteination and detection into one reagent. DTNB reacts with reduced glutathione to form a yellow product. The optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample. The optimized formulation has a long shelf life and is completely free of interference due to sample turbidity.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1