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Documentos Principais

CS0260

Sigma-Aldrich

Glutathione Assay Kit

sufficient for 700 assays

Sinônimo(s):

GSH Quantification Test

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 700 assays

Nível de qualidade

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Informações sobre genes

human ... GSR(2936)

Descrição geral

The kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in a cell and tissue extracts and in red blood cells or plasma. The sample is first deproteinized with the 5% 5-sulfosalicylic acid solution. Glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of 5,5′-dithiobis-(2-nitrobenzoic) acid (DTNB) to TNB. The oxidised glutathione formed is recycled by glutathione reductase and NADPH. The product, TNB, is assayed colorimetrically at 412 nm.

Aplicação

Glutathione Assay Kit has been used to measure total glutathione concentration in glutathione assay. It also has been used to determine different oxidative stress parameters in rats.

Ações bioquímicas/fisiológicas

Glutathione (GSH) functions as a cofactor for several antioxidant enzymes. It is involved in the regeneration of vitamins C and E. It modulates cellular proliferation and apoptosis. GSH is crucial for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene are associated with Alzheimer′s, Parkinson′s and Huntington′s diseases. It is also linked to cystic fibrosis, immune diseases and cardiovascular diseases.
Reduced glutathione (GSH), a tripeptide (g-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues

Características e benefícios

  • A quick and simple method
  • The kit contains all the reagents required for a colorimetric assay of total glutathione (oxidised + reduced GSSG + GSH)
  • The kit includes a deproteination reagent for biological samples

Somente componentes do kit

Nº do produto
Descrição

  • Assay Buffer 5x 30 mL

  • DTNB 8 mg

  • DMSO 7.5 mL

  • Glutathine Reductase 20 U

  • L-Glutathione Reduced 300 μg

  • NADPH 25 mg

  • 5-Sulfosalicylic Acid 2.5 g

produto relacionado

Nº do produto
Descrição
Preços

Pictogramas

Health hazardCorrosion

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Ponto de fulgor (°F)

188.6 °F

Ponto de fulgor (°C)

87 °C


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