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Documentos Principais

CS0410

Sigma-Aldrich

Glutathione-S-Transferase (GST) Assay Kit

sufficient for 500 multiwell tests

Sinônimo(s):

GST Enzyme Activity Kit

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About This Item

Número CE:
Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 500 multiwell tests

Nível de qualidade

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Informações sobre genes

Descrição geral

The Glutathione S-Transferase (GST) Assay Kit is designed to measure total GSTactivity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in plasma
and erythrocyte lysates

Aplicação

The Glutathione S-Transferase Assay kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB), which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.

Ações bioquímicas/fisiológicas

Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification.

Somente componentes do kit

Nº do produto
Descrição

  • Dulbecco's Phosphate Buffered Saline 100 mL

  • L-Glutathione Reduced 1 g

  • GST (Control) .1 mL

  • Sample Buffer 5 mL

  • Substrate (CDNB) 1.2 mL

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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