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Documentos Principais

MAK062

Sigma-Aldrich

IDH Activity Assay Kit

sufficient for 100 colorimetric tests

Sinônimo(s):

Isocitrate Dehydrogenase Activity Assay

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

método de detecção

colorimetric

doença(s) relevante(s)

cancer

temperatura de armazenamento

−20°C

Informações sobre genes

human ... IDH1(3417) , IDH2(3418) , IDH3B(3420) , IDH3G(3421)
mouse ... IDH1(15926) , IDH2(269951) , IDH3B(170718) , IDH3G(15929)
rat ... IDH1(24479) , IDH2(361596) , IDH3B(94173) , IDH3G(25179)

Descrição geral

Isocitrate dehydrogenase (IDH) catalyzes the conversion of isocitrate to α-ketoglutarate. In eukaryotes, there are three isozymes of IDH, the mitochondrial IDH2 and IDH3, and the cytoplasmic/ peroxisomal IDH1. All three IDH family members require the presence of a divalent cation (Mg2+ or Mn2+) and either the electron-accepting cofactor NADP+ (IDH1 and IDH2) or NAD+ (IDH3) for their enzymatic activity. IDH1 and IDH2 mutations resulting in neomorphic enzymatic activity are found in certain cancers such as glioblastoma, acute myeloid leukemia, and colon cancer. This neoactivity shows a change in the substrate specificity resulting in the conversion of α-ketoglutarate to 2-hydroxyglutarate. Mutations in IDH family members are also associated with Ollier disease and Maffucci syndrome.

Aplicação

IDH Activity Assay Kit has been used to determine the activity of isocitrate dehydrogenase in samples.

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

Suitable for the measurement of isocitrate dehydrogenase (IDH) (NAD+ - dependent, NADP+ -dependent or both IDHs) activity in biological samples including tissue, cells and serum

Princípio

The Isocitrate Dehydrogenase Activity Assay kit provides a simple and direct procedure for measuring NADP+-dependent, NAD+-dependent, or both NADP+ and NAD+-dependent IDH activity in a variety of samples. IDH activity is determined using isocitrate as the substrate in an enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of IDH is the amount of enzyme that will generate 1.0 μmole of NADH or NADP per minute at pH 8.0 at 37 °C.

Aplicação

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Pictogramas

Health hazardCorrosion
GHS08,GHS05

Palavra indicadora

Danger

Frases de perigo

H314,H334

Classificações de perigo

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

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Certificados de análise (COA)

Lot/Batch Number
7L10K07560
7J08K07560
7G15K07560
7D05K07560
7B22K07560

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Protocolos

Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers

We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.

Chromatograms

application for HPLC

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