MAK178
Enolase Activity Assay Kit
Sufficient for 100 Colorimetric or Fluorometric tests
Sinônimo(s):
Enolase Detection Kit
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Produtos recomendados
método de detecção
colorimetric
fluorometric
doença(s) relevante(s)
immunological diseases; cardiovascular diseases
temperatura de armazenamento
−20°C
Informações sobre genes
human ... EBI4(387712) , ENO1(2023) , ENO2(2026)
mouse ... EBI4(226265) , ENO1(13806) , ENO2(13807)
rat ... EBI4(292138) , ENO1(24333) , ENO2(24334)
Descrição geral
Enolase is a multifunctional glycolytic enzyme that catalyzes the conversion of D-2-phosphoglycerate to phospho(enol)pyruvate (PEP) and water. It can function as a plasminogen receptor in endothelial, epithelial, and hematopoietic cells and hence, may be involved in fibrinolytic and intravascular systems. It is also known to act as a heat shock protein, which may have implications in transcriptional and pathological functions. Enolase has been implicated in autoimmune and systemic diseases. Furthermore; serum neuron-specific enolase is known to function as a predictor of patient outcome post cardiac arrest. Thus enolase assays can be used for studying cellular functions like carbohydrate metabolism, transcription and other pathophysiological processes.
Aplicação
Enolase Activity Assay Kit has been used to measure enolase activity.
Adequação
Suitable for the detection of enolase activity in biological samples.
Princípio
The Enolase activity is determined by a coupled enzyme assay in which D-2-phosphoglycerate is converted to PEP, resulting in the formation of an intermediate that reacts with a peroxidase substrate, generating a colorimetric (570 nm) or fluorometric (λex = 535/λem = 587 nm) product proportional to the enolase activity present. One milliunit of enolase is the amount of enzyme that will generate 1.0 nmole of H2O2 per minute at pH 7.2 at 25 °C.
Somente componentes do kit
Nº do produto
Descrição
- Enolase Assay Buffer
- Peroxidase Substrate, in DMSO
- Enolase Substrate Mix
- Enolase Converter
- Enolase Developer
- Enolase Positive Control
- Hydrogen Peroxide Standard, 0.88 M
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
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