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Documentos Principais

MAB995

Sigma-Aldrich

Anti-Bacterial Peptidoglycan Antibody, clone 3F6B3

ascites fluid, clone 3F6B3, Chemicon®

Sinônimo(s):

PG

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

3F6B3, monoclonal

reatividade de espécies

bacteria

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable

Isotipo

IgG1

Condições de expedição

wet ice

Especificidade

Specific for the three-dimensional polymer complex structure of PG. The epitopes appear to consist of discontinuous glycan and/or amino acid residues, and have not been fully defined. In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors: muramyldipeptide, N-acetylglucosamine (NAG), chitin (polymer of NAG) and acid-hydrolyzed chitin (NAG oligomers).

Imunogênio

Insoluble PG obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.

Aplicação

May be used in solid-phase or competitive ELISA, immunodot-blot and immunocytochemistry formats. Although PG is a bacterial surface component, pre-treatments, such as treatment with strong acid (25mM HCl), for Gram-positive bacteria, or with a detergent, such as SDS or Triton-X (1% in PBS), for Gram-negative bacteria, may be necessary to expose the epitopes.

Optimal working dilutions must be determined by end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
This Anti-Bacterial Peptidoglycan Antibody, clone 3F6B3 is validated for use in DB, ELISA, IC for the detection of Bacterial Peptidoglycan.

forma física

Ascites fluid, no preservatives. Liquid.

Armazenamento e estabilidade

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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T Wagner et al.
Journal of proteomics, 187, 28-38 (2018-06-02)
Enterococcus faecium is a commensal but also a bacteremia causing pathogen, which is inherently resistant to several antimicrobials and has a great ability to acquire new traits. Bacterial membrane vesicles (MVs) are increasingly recognized as a mode of cell-free communication
Judith Miklossy
Journal of Alzheimer's disease : JAD, 53(4), 1459-1473 (2016-06-18)
It has long been known that spirochetes form clumps or micro colonies in vitro and in vivo. Cortical spirochetal colonies in syphilitic dementia were considered as reproductive centers for spirochetes. Historic and recent data demonstrate that senile plaques in Alzheimer's
Emily Eckman et al.
Journal of molecular medicine (Berlin, Germany) (2021-06-09)
A panel of 10 IgG enzyme-linked immunosorbent assays (ELISAs) were developed for the detection of anti-microbial immune responses in the cerebrospinal fluid (CSF) of patients with demyelinating diseases (DD). The anti-microbial ELISA assays follow on prior human brain tissue RNA
Brain microbial populations in HIV/AIDS: ?-proteobacteria predominate independent of host immune status.
Branton, WG; Ellestad, KK; Maingat, F; Wheatley, BM; Rud, E; Warren, RL; Holt et al.
Testing null
Judith Miklossy et al.
Journal of neuroinflammation, 5, 40-40 (2008-09-27)
The long latent stage seen in syphilis, followed by chronic central nervous system infection and inflammation, can be explained by the persistence of atypical cystic and granular forms of Treponema pallidum. We investigated whether a similar situation may occur in

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