MAK118
Invertase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Sinônimo(s):
β-fructofuranosidase Assay Kit
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Produtos recomendados
uso
sufficient for 100 colorimetric or fluorometric tests
aplicação(ões)
cosmetics
environmental
food and beverages
método de detecção
colorimetric
fluorometric
temperatura de armazenamento
−20°C
Descrição geral
Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.
Aplicação
Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.
Características e benefícios
Compatible with high-throughput handling systems.
Adequação
Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.
Princípio
In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.
Código de classe de armazenamento
10 - Combustible liquids
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In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play
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