MAK009
Amylase Activity Assay Kit
sufficient for 100 colorimetric tests
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Produtos recomendados
uso
sufficient for 100 colorimetric tests
método de detecção
colorimetric
doença(s) relevante(s)
endocrinological disorders, diabetes; gastrointestinal diseases
temperatura de armazenamento
−20°C
Informações sobre genes
human ... AMY1A(278)
Descrição geral
The new Amylase Assay Kit, MAK478, is now available! Amylase enzymes are glycoside hydrolases, which cleave glucan linkages in polysaccharides such as starch or glycogen to release polysaccharide molecules. α-Amylase is found in animals, plants, and many fungi. In mammals, α-amylase is the predominant form of amylase and is one of the major enzymes of dietary digestion. Both the activity and the stability of α-amylase is dependent on calcium ions. In humans, α-amylase levels are highest in pancreatic and salivary secretions. Increased blood and urine levels of amylase occur due to pancreatic disorders and salivary trauma. Increased salivary amylase activity is also a marker for increased sympathetic nervous system activity.
Aplicação
Amylase Activity Assay Kit has been used to determine the activity of amylase.
Adequação
Suitable for the quantification of amylase activity in a variety of biological samples.
Princípio
The Amylase Activity Assay kit quantifies amylase activity in a variety of biological samples. The assay is quick, convenient, and sensitive. In this kit, amylase activity is determined using a coupled enzymatic assay, which results in a colorimetric (405 nm) product, proportional to the amount of substrate, ethylidene-pNP-G7, cleaved by the amylase. One unit is the amount of amylase that cleaves ethylidene-pNP-G7 to generate 1.0 μmole of p-nitrophenol per minute at 25 °C.
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Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
10 - Combustible liquids
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Artigos
Assay protocol for the colorimetricdetection of amylase activity in biological samples using the Amylase Activity assay kit.
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