G5003

α-Glucosidase from Saccharomyces cerevisiae

Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)

• Sinônimo(s): α-D-Glucosidase, α-D-Glucoside glucohydrolase, Maltase from yeast
• Número CAS: 9001-42-7
• Número da licença da enzima: 3.2.1.20 (BRENDA, IUBMB)
• Número CE: 232-604-7
• Número MDL: MFCD00081321
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• tipo: Type I
• Nível de qualidade: 200
• forma: lyophilized powder
• atividade específica: ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
• contém: phosphate buffer salts and EDTA as balance
• composição: Protein, ≥50%
• características do produto alternativo mais ecológico: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• nº de adesão UniProt: P38158; P53341
• atividade externa: β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%
• categoria alternativa mais ecológica: , Enabling
• doença(s) relevante(s): diabetes (management of non-insulin-dependent diabetes mellitus)
• temperatura de armazenamento: −20°C
• Informações sobre genes: bakers yeast ... MAL12(853209) , MAL32(852602)

Descrição

Descrição geral

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Aplicação

α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.

For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters

Ações bioquímicas/fisiológicas

α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus.

Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.

Embalagem

Sold on basis of p-nitrophenyl α-D-glucoside units.

Definição da unidade

One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.

Nota de análise

Protein determined by biuret.

Outras notas

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)