M9309
McCoy′s 5A Medium
With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sinônimo(s):
5A basal medium
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Nome do produto
McCoy′s 5A Medium, Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
esterilidade
sterile-filtered
Formulário
liquid
técnica(s)
cell culture | mammalian: suitable
Impurezas
endotoxin, tested
componentes
NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
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Descrição geral
The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.
Aplicação
McCoy′s 5A Medium has been used:
- to culture HCT-116?cells
- to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
- to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies
Ações bioquímicas/fisiológicas
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
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Descrição
Preços
suplemento
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Descrição
Preços
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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