M4892

McCoy′s 5A Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• Sinônimo(s): 5A basal medium
• Número MDL: MFCD00217560
• Código UNSPSC: 12352207

Propriedades

• Nível de qualidade: 500
• forma: powder
• técnica(s): cell culture | mammalian: suitable
• componentes: phenol red: 0.011 g/L; L-glutamine: 0.21915 g/L; glucose: 3.0 g/L (Dextro); NaHCO₃: no
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Aplicação

McCoy′s 5A Medium has been used for culturing:

• human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
• human colorectal HCT116 cells to study miR-224 over-expression in cancer
• osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Ações bioquímicas/fisiológicas

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Quantidade

Formulated to contain 11.9 grams of powder per liter of medium.

Reconstituição

Supplement with 2.2 g/L sodium bicarbonate.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3