M4892
McCoy′s 5A Medium
Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Fabricação farmacêutica
Sinônimo(s):
5A basal medium
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About This Item
Produtos recomendados
Nível de qualidade
forma
powder
técnica(s)
cell culture | mammalian: suitable
componentes
phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
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Descrição geral
McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.
Aplicação
McCoy′s 5A Medium has been used for culturing:
- human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
- human colorectal HCT116 cells to study miR-224 over-expression in cancer
- osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media
Ações bioquímicas/fisiológicas
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
Quantidade
Formulated to contain 11.9 grams of powder per liter of medium.
Reconstituição
Supplement with 2.2 g/L sodium bicarbonate.
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Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
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