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M9163

Sigma-Aldrich

Medium 199

With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

Sinônimo(s):

M199 Medium, TCM 199

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nome do produto

Medium 199, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

esterilidade

sterile-filtered

Formulário

liquid

concentração

10 ×

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Reconstituição

Supplement with 0.1 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Medium 199 Formulation

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