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Key Documents

M8645

Sigma-Aldrich

Anti-Mouse IgG (whole molecule) antibody produced in goat

affinity isolated antibody, lyophilized powder

Sinônimo(s):

Goat Anti-Mouse IgG, Goat IgG Antibody

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

goat

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

forma

lyophilized powder

técnica(s)

Ouchterlony double diffusion: suitable

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Imunogênio

IgG purified from pooled normal mouse serum.

Aplicação

Anti-mouse IgG (whole molecule) antibody (diluted 1:20) can be used in Indirect immunofluorescence. It can also be used as a secondary antibody in western blotting to release NC1 domain of type IV collagen. Further, it can also be used in enzyme immunoassays (50 μgram) .
Anti-mouse IgG (whole molecule) antibody (diluted 1:20) can be used in:
  • indirect immunofluorescence
  • western blotting to release NC1 domain of type IV collagen
  • enzyme immunoassays (50 μgram)

Ações bioquímicas/fisiológicas

IgG regulates several functions such as phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-mouse IgG (whole molecule) antibody can be used in T cell proliferation assays. It can also be used in Ouchterlony Double Diffusion and immunoelectrophoresis. Goat anti- mouse IgG (whole molecule) antibody reacts specifically with mouse IgG subclasses IgG1, IgG2a, IgG2b and IgG3. The product has also shown no reactivity to human serum proteins.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Outras notas

Antibody adsorbed with human serum proteins

forma física

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Reconstituição

Reconstitute with 1 mL of 0.135 M sodium chloride.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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