L4025
Liticase
lyophilized powder, ≥200 units/mg solid
Sinônimo(s):
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase, Bacterial lyticase, Lysing enzyme
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About This Item
Produtos recomendados
fonte biológica
bacterial (Arthrobacter luteus)
Nível de qualidade
forma
lyophilized powder
atividade específica
≥200 units/mg solid
técnica(s)
cell based assay: suitable
adequação
suitable for cell lysis
aplicação(ões)
diagnostic assay manufacturing
temperatura de armazenamento
−20°C
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Aplicação
Lyticase from Arthrobacter luteus has been used for resuspending cells to extract high-molecular weight DNA for polymerase chain reaction (PCR) fingerprint analyses.
Lyticase from Arthrobacter luteus has been used:
- as a component in spheroplasting buffer to prepare yeast nuclear extracts
- for digestion in sample preparation for β-glucan enzymatic assay using yeast
- to lyse cells for RNA isolation
Ações bioquímicas/fisiológicas
Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units.
Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. It is useful with yeast cells such as e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.
A liticase hidrolisa a poli-β(1→3)-glicose, como glucana da parede celular de levedura.
Definição da unidade
Uma unidade produzirá uma ΔA800 de 0,001 por minuto em pH 7,5 a 25 °C, usando uma suspensão de levedura como substrato em uma mistura de reação de 3 ml.
Outras notas
Veja mais informações sobre enzimas para análise de carboidratos complexos em www.sigma-aldrich.com/enzymeexplorer
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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Protocolos
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
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