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H9394

Sigma-Aldrich

Solução salina balanceada de Hank

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Sinônimo(s):

HBSS

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nível de qualidade

400

esterilidade

sterile-filtered

Formulário

liquid

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

Condições de expedição

ambient

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Categorias relacionadas

Descrição geral

Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem.

Aplicação

Hanks′ Balanced Salt solution has been used:
  • as a component of the culture medium for culturing of mouse cortical slices
  • as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs)
  • for in situ perfusions of mice liver

Ações bioquímicas/fisiológicas

Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
RNBL8854
RNBL5851
RNBL1816
RNBL0992
RNBK8408

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Hanks’ Balanced Salts Modified, without calcium chloride, magnesium sulfate, phenol red and sodium bicarbonate, powder, suitable for cell culture

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Solução salina tamponada com fosfato Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

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Protocolos

Removal of Adherent Cells from a Culture Surface Using Trypsin

Trypsin may be used to remove adherent cells from a culture surface. Cells are most commonly removed from the culture substrate by treatment with trypsin or trypsin/EDTA solutions.

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

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