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Documentos Principais

H9269

Sigma-Aldrich

Solução salina balanceada de Hank

With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sinônimo(s):

HBSS

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nível de qualidade

esterilidade

sterile-filtered

Formulário

liquid

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

NaHCO3: 0.35 g/L
phenol red: 0.011 g/L
glucose: 1.0 g/L (Dextro)

Condições de expedição

ambient

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Descrição geral

Formulation: click here.

Aplicação

Hanks′ balanced salt solution has been used:
  • in starvation experiments for mammalian cells
  • to collect and maintain brain pieces for primary glial cell culture
  • to wash femoral tissue and bone marrow (BM) to culture mesenchymal stem cells

Ações bioquímicas/fisiológicas

Balanced salt solution (BSS) in cell culture is multifaceted and has various functions. It serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. BSS provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It combines with carbohydrates, such as glucose to act as the principal energy source for cell metabolism. BSS also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Protocolos

The 96 Well 3D Spheroid BME Cell Invasion Assay utilizes 3D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells.

Cultrex® 3-D Spheroid Fluorometric Proliferation/Viability Assay Protocol

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