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Key Documents

H4641

Sigma-Aldrich

Solução salina balanceada de Hank

Without calcium chloride, magnesium sulfate and sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

Sinônimo(s):

Hanks’ Balanced Salt solution, HBSS

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nível de qualidade

esterilidade

sterile-filtered

forma

liquid

concentração

10 ×

técnica(s)

cell culture | mammalian: suitable

componentes

phenol red: yes

Condições de expedição

ambient

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Descrição geral

Balanced salt solution (BSS) is crucial in cell culture as it serves as an irrigating, transporting, and diluting agent to maintain the intra- and extracellular osmotic balance and also provides energy for cell metabolism. It provides water and bulk inorganic ions to the cells to maintain the normal cell metabolism. BSS plays a role in the buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Aplicação

Hanks′ Balanced Salt Solution 10x has been used:

  • as a diluting agent during the Chinese hamster ovary cell line detachment from growth medium
  • to wash the biopsy specimens of lamina propria lymphocytes (LPLs)
  • as a wash solution to wash the fish cell line during removal from growth medium

Reconstituição

Supplement with 0.35 g/L sodium bicarbonate at 1X.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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