H8666

Transforming Growth Factor-β2 human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Sinônimo(s): TGF-β2
• Número MDL: MFCD00163957
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• product name: Transforming Growth Factor-β2 human, TGF-β2, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• fonte biológica: human
• Nível de qualidade: 200
• recombinante: expressed in HEK 293 cells
• Ensaio: ≥95% (SDS-PAGE)
• forma: lyophilized powder
• potência: ≤0.5 ng/mL EC₅₀
• qualidade: endotoxin tested
• peso molecular: dimer 25 kDa (non-glycosylated)
• embalagem: pkg of 5 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• técnica(s): cell culture | mammalian: suitable
• Impurezas: ≤1 EU/μg
• nº de adesão UniProt: P61812
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... TGFB2(7042)

Descrição

Descrição geral

Transforming growth factor-β2 (TGF-β2) belongs to the TGF-β superfamily. All threes isoforms (TGF-β1, TGF-β2 and TGF-β3) are known to share similar structure and function. The TGFB2 gene is mapped to human chromosome 1q41 and is localized to extracellular matrix. The active form of TGF-β precursor is produced upon proteolytic cleavage. TGF-β1 and TGF-β2 have 70% amino acid sequence identity.

Ações bioquímicas/fisiológicas

TGF-β2 is important for immune homeostasis by balancing lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis. TGF-β2 is crucial in cell growth, differentiation, and survival. TGF-β2 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-β is associated with the process of tumor development, progression and metastasis. TGF-β2 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β2 inhibits antitumor action of NK (natural killer) cells, T-cells, macrophages, monocytes and neutrophils. TGF-β is also associated with inflammation and wound healing. The TGF-β gene is overexpressed in glioma tumors and also in colon cancer, gastric cancer and cervical lesions.

Transforming growth factor-β2 (TGF-β2), like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

Nota de preparo

HumanKine TGF-β2 is expressed in human HEK 293 cells as a mature, non-glycosylated, disulfide-linked homodimer with a predicted molecular mass of approx. 25 kDa.

Nota de análise

The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Informações legais

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable