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H5000

Sigma-Aldrich

Hexokinase from Saccharomyces cerevisiae

Type III, lyophilized powder, ≥25 units/mg protein (biuret)

Sinônimo(s):

ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast

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About This Item

Número CAS:
9001-51-8
Número da licença da enzima:
2.7.1.1 (BRENDA, IUBMB)
Número CE:
232-611-5
Número MDL:
MFCD00131321
Código UNSPSC:
12352204
eCl@ss:
32160410

tipo

Type III

forma

lyophilized powder

atividade específica

≥25 units/mg protein (biuret)

peso molecular

dimer 110 kDa

nº de adesão UniProt

P04806
P04807

atividade externa

glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%

temperatura de armazenamento

−20°C

Informações sobre genes

bakers yeast ... HXK1(850614) , HXK2(852639)

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Ações bioquímicas/fisiológicas

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)

Definição da unidade

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.

forma física

Lyophilized powder containing approx. 15% sodium citrate

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

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