H1404

Hepatocyte Growth Factor human

>95% (SDS-PAGE), recombinant, expressed in Baculovirus infected High-5 cells, lyophilized powder, suitable for cell culture

• Sinônimo(s): HGF, Hepatopoietin A, Scatter Factor
• Número CE: 214-275-1
• Número MDL: MFCD00217422
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• Nome do produto: Hepatocyte Growth Factor human, HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture
• fonte biológica: human
• Nível de qualidade: 200
• recombinante: expressed in Baculovirus infected High-5 cells
• Ensaio: >95% (SDS-PAGE)
• Formulário: lyophilized powder
• potência: 1.0-20.0 ng/mL
• qualidade: endotoxin tested
• peso molecular: ~80 kDa
• embalagem: pkg of 5x5 μg; pkg of 5 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <1.0 EU/μg
• nº de adesão UniProt: P14210
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... HGF(3082)

Descrição

Ações bioquímicas/fisiológicas

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Outras notas

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

forma física

Lyophilized from a 0.2 μm filtered solution in 20 mM NaH₂PO₄ and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF

Nota de preparo

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.

Nota de análise

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves