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Documentos Principais

GE17-5161-01

HiTrap® SP XL

Cytiva 17-5161-01, pack of 5 × 5 mL

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

sulphopropyl

descrição

Ion Exchanger Type (value)

embalagem

pack of 5 × 5 mL

fabricante/nome comercial

Cytiva 17-5161-01

Parâmetros

<20 mL/min flow rate
42 psi

tamanho do leito

16 mm × 25 mm

volume do leito

5 mL

D.I. da coluna

16 mm

Matriz

6% cross-linked agarose with dextran surface extenders

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning

3-14

Faixa de trabalho

4-13

adequação

suitable for bioprocess medium

Categorias relacionadas

Descrição geral

HiTrap® SP XL are strong cation exchangers prepacked with SP Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

Aplicação

Q Sepharose XL and SP Sepharose XL media have long chains of Dextran coupled to a robust, 6% highly cross-linked Agarose matrix. The Dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

Características e benefícios

  • High binding capacities with high fLow rates.
  • Convenient and affordable for fast, easy separations either alone or connected in series.
  • Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA® design.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


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Certificados de análise (COA)

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Artigos

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolos

This page covers how and when to use Sepharose XL media for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

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