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G6784

Sigma-Aldrich

ʟ-Glutamine–Penicillin–Streptomycin

solution, suitable for cell culture, BioReagent

Sinônimo(s):

Pen/Strep, Penicillin/Streptomycin 

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.76

Nome do produto

L-Glutamine–Penicillin–Streptomycin solution, L-glutamine 200 mM, streptomycin 10 mg/mL, penicillin 10,000 units, 0.1 μm filtered, BioReagent, suitable for cell culture

fonte biológica

synthetic

Nível de qualidade

descrição

stabilized

esterilidade

0.1 μm filtered

linha de produto

BioReagent

Formulário

liquid

composição

L-glutamine, 200 mM
penicillin, 10,000 units
streptomycin, 10 mg/mL

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

espectro de atividade do antibiótico

Gram-negative bacteria
Gram-positive bacteria

Modo de ação

cell wall synthesis | interferes
protein synthesis | interferes

temperatura de armazenamento

−20°C

Aplicação

This antibiotic solution is used to reduce or eliminate bacterial contamination in primary cell cultures. It is recommended for us at 10 mL/L.

Ações bioquímicas/fisiológicas

Mode of Action: The product acts in three ways: Glutamine is an essential energy source for most mammalian cells in culture due to its amino acid carbon skeleton. Penicillin inhibits bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria.

Componentes

This solution is formulated to contain 200 mM L-glutamine, 10,000 units of Penicillin and 10 mg/mL of streptomycin, solubilized in a proprietary buffer.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)


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Zhengliang L Wu et al.
Glycobiology, 30(7), 454-462 (2020-01-04)
Cells are covered with glycans. The expression and distribution of specific glycans on the surface of a cell are important for various cellular functions. Imaging these glycans is essential to aid elucidation of their biological roles. Here, utilizing methods of
N Massa et al.
Canadian journal of microbiology, 64(10), 647-663 (2018-05-11)
Candida glabrata is an opportunistic pathogen, associated with endocarditis, meningitis, and disseminated disease, and also with complicated vaginitis. Essential oils derived from aromatic plants are known in traditional medicine as antimicrobial agents and have antifungal properties. The aim of this
Nikoletta Sztán et al.
Reproduction, fertility, and development, 29(11), 2206-2216 (2017-03-28)
The conservation of genetic resources of avian species has become increasingly important over the past decade. The aim of the present study was to develop a genome preservation technique for the Hungarian goose Anser anser domestica. To this end, we
Chi-Fei Wang et al.
Anesthesia and analgesia, 107(4), 1397-1405 (2008-09-23)
Bulleyaconitine A (BLA) is an analgesic and antiinflammatory drug isolated from Aconitum plants. BLA has several potential targets, including voltage-gated Na+ channels. We tested whether BLA elicited long-lasting cutaneous analgesia, when co-injected with lidocaine and epinephrine, as a model for
Miriam Gonzalez Gonzalez et al.
Journal of immunological methods, 457, 73-77 (2018-02-25)
Standard cell culturing on plastic plates (two dimensional (2D) cultures) does not represent the actual microenvironment where cells reside in tissues. The three dimensional (3D) systems, composed of extracellular matrix and/or pure amino acids which form a scaffold for cells

Artigos

Importance and uses of glutamine in hybridoma and mammalian cell culture

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