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G4913

Sigma-Aldrich

Anti-GAD2(GAD67) Antibody

rabbit polyclonal

Sinônimo(s):

Anti-GAD 65

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About This Item

Número MDL:
MFCD03455043
Código UNSPSC:
12352203
NACRES:
NA.43

product name

Anti-Glutamic Acid Decarboxylase 65 (514-530) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

fonte biológica

rabbit

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

IgG fraction of antiserum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

reatividade de espécies

mouse, pig, human, rat

técnica(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using tissue sections of rat pancreas
microarray: suitable
western blot: 1:4,000 using rat brain extract

nº de adesão UniProt

Q05329

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)

Descrição geral

GAD is predominantly expressed in the central nervous system (CNS) and pancreatic islets. It is also expressed in testis, oviduct and ovary. GAD exists in two isofoms, GAD 65 and GAD 67. GAD65 is an ampiphilic, membrane-anchored protein, (585 amino acid residues) and is encoded on human chromosome 10.

Especificidade

Reacts specifically with GAD 65 (65 kDa) from brain.

Imunogênio

synthetic peptide corresponding to the C-terminal region of human GAD 65 (amino acids 514-530). The sequence is identical in mouse GAD 65 and highly conserved in rat and pig GAD 65 but not found in GAD 67.

Aplicação

Anti-Glutamic Acid Decarboxylase 65 antibody produced in rabbit is suitable as a primary antibody for the determination of expression of GAD 65 isoform in putative P boutons in rat and mouse at a working dilution of 1:5000 and in lysates prepared from rat testis at different developmental ages and rat cerebellum by immunoblotting.
It is also suitable for immunohistochemistry at a working dilution of 1:500 using formalin-fixed, paraffin-embedded sections of rat pancreas and for microarray.

Ações bioquímicas/fisiológicas

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L-glutamate to γ-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. It has been identified as an autoantigen in insulin-dependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS) and may serve as a marker in the early stages of IDDM. GAD 65 synthesizes transmitter GABA for vesicular release.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Satoko Matsukawa et al.
Journal of biochemistry, 142(5), 633-638 (2007-10-30)
Human peripheral blood leucocyte was examined for the expression of glutamate decarboxylase (GAD). Peripheral blood from healthy individuals was fractionated into polynuclear leucocytes and mononuclear leucocytes. Cell lysate from the mononuclear leucocytes was analysed by SDS-PAGE and Western blot. With
Safa Shehab et al.
The European journal of neuroscience, 22(6), 1431-1444 (2005-09-30)
Pharmacological manipulation of the ventrolateral pontine reticular formation (vlPRF) of rats has an anticonvulsant effect in the maximal electroshock model of epilepsy. This study presents three anatomical experiments that determine the efferent projections from this region likely to mediate this
Haixiong Liu et al.
Acta biochimica et biophysica Sinica, 41(7), 545-553 (2009-07-07)
Glutamate decarboxylase 65 (GAD65) produces gamma-aminobutyric acid, the main inhibitory neurotransmitter in adult mammalian brain. Previous experiments, performed in brain, showed that GAD65 gene possesses two TATA-less promoters, although the significance is unknown. Here, by rapid amplification of cDNA ends
N J Tillakaratne et al.
Journal of neurochemistry, 58(2), 618-627 (1992-02-01)
gamma-Aminobutyric acid (GABA) and its synthetic enzyme, glutamate decarboxylase (GAD), are not limited to the nervous system but are also found in nonneural tissues. The mammalian brain contains at least two forms of GAD (GAD67 and GAD65), which differ from
M G Erlander et al.
Neurochemical research, 16(3), 215-226 (1991-03-01)
Studies of the GABA-synthetic enzyme glutamate decarboxylase (glutamic acid decarboxylase; GAD; E.C.4.1.1.15) began in 1951 with the work of Roberts and his colleagues. Since then, many investigators have demonstrated the structural and functional heterogeneity of brain GAD. At least part
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