G3153
β-Galactosidase from Escherichia coli
lyophilized powder, ≥500 units/mg protein
Sinônimo(s):
β-D-Galactoside galactohydrolase, Lactase
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About This Item
Produtos recomendados
recombinante
expressed in E. coli
Nível de qualidade
Formulário
lyophilized powder
atividade específica
≥500 units/mg protein
peso molecular
465 kDa
temperatura de armazenamento
−20°C
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Descrição geral
β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.
Aplicação
β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).
β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.
Ações bioquímicas/fisiológicas
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.
Embalagem
Package size based on protein content
propriedades físicas
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Definição da unidade
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
forma física
Stabilized with phosphate buffer and sucrose
Inibidor
substrato
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Nucleic Acids Research, 20(12), 3254-3254 (1992)
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