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E4913

Sigma-Aldrich

Plant/Fungi RNA Purification Kit

greener alternative

sufficient for 50 purifications

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About This Item

Código UNSPSC:
41105501
NACRES:
NA.52

uso

sufficient for 50 purifications

Nível de qualidade

100

características do produto alternativo mais ecológico

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

categoria alternativa mais ecológica

, Aligned

Descrição geral

Norgen Biotek′s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including viral RNA, from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples.

Aplicação

For the rapid purification of total RNA (including microRNA) from plants and fungi.
Purified RNA is of the highest quality and can be used in a number of downstream applications, including:
  • qRT-PCR
  • Reverse transcription PCR
  • Northern blotting
  • RNAse protection
  • Primer extension
  • Expression array assays
  • Next Generation Sequencing

Características e benefícios

  • Isolate RNA from a wide range of plant and filamentous fungi samples.
  • Robust lysis buffer is suitable for challenging samples such as pine needle or grape leaf.
  • Isolate a diversity of RNA species from large mRNA, microRNA, and viral RNA.
  • Purification of high yield and quality RNA.
  • No phenol/chloroform extraction required.
  • Liquid nitrogen is not required for homogenization

Outras notas

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

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GHS07,GHS09

Palavra indicadora

Warning

Classificações de perigo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Biochemical and biophysical research communications, 396(2), 187-192 (2010-03-25)
DNA methyltransferase2 (DNMT2) is always deemed to be enigmatic, because it contains highly conserved DNA methyltransferase motifs but lacks the DNA methylation catalytic capability. Here we show that Arabidopsis DNA methyltransferase2 (AtDNMT2) is localized in nucleus and associates with histone
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MicroRNAs (miRNAs) are important regulators of many cellular processes and exist in a wide range of eukaryotes. High-throughput sequencing is a mainstream method of miRNA identification through which it is possible to obtain the complete small RNA profile of an
Hila Yaffe et al.
BMC research notes, 5, 45-45 (2012-01-21)
Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues
Kobi Buxdorf et al.
Plant molecular biology, 84(1-2), 37-47 (2013-08-15)
The cuticle plays an important role in plant interactions with pathogens and with their surroundings. The cuticle acts as both a physical barrier against physical stresses and pathogens and a chemical deterrent and activator of the plant defense response. Cuticle
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Protocolos

Plant RNA/DNA Purification Kit

The Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants.

Plant/Fungi DNA Isolation Kit Protocol

The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.

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