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D5637

Sigma-Aldrich

3,3′-Diaminobenzidine tetrahydrochloride hydrate

≥96%

Sinônimo(s):

(1,1′-Biphenyl)-3,3′,4,4′-tetramine tetrahydrochloride dihydrate

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About This Item

Fórmula linear:
C12H14N4 · 4HCl · xH2O
Número CAS:
868272-85-9
Número CE:
231-018-9
Número MDL:
MFCD08273058
Código UNSPSC:
12171500
ID de substância PubChem:
57654109
NACRES:
NA.47

Nível de qualidade

200

Ensaio

≥96%

forma

powder, crystals or chunks

técnica(s)

titration: 96-104% (with NaOH)

cor

white to light brown, and Faint Red and Faint Grey to Light Grey

pf

280 °C

solubilidade

H2O: 50 mg/mL

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

chave InChI

DXWSCXIZIHILNP-UHFFFAOYSA-N

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Descrição geral

3,3′-Diaminobenzidine tetrahydrochloride hydrate is a chromogen commonly used in immunohistochemistry and other staining techniques to visualize the presence of antigens or antibodies in biological samples. DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.

Aplicação

3,3′-Diaminobenzidine tetrahydrochloride hydrate is suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).

Ações bioquímicas/fisiológicas

In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palavra indicadora

Danger

Frases de perigo

H302,H319,H341,H350

Classificações de perigo

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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