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Key Documents

D5167

Sigma-Aldrich

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse

clone 70.1, ascites fluid

Sinônimo(s):

Anti-CILD1, Anti-DIC1, Anti-ICS1, Anti-PCD

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

conjugado

unconjugated

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

70.1, monoclonal

contém

15 mM sodium azide

reatividade de espécies

marsupial, hamster, human, Indian muntjac (Muntiacus muntjac vaginalis), chicken, rat, frog

técnica(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:2,000 using a chick brain extract

Isotipo

IgM

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

Descrição geral

Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus. Monoclonal anti-dynein (Intermediate Chain) antibody can be used for localization of dynein intermediate chain using different immunochemical assays like ELISA, immunoblotting and dot blot. Mouse anti- dynein (Intermediate Chain) antibody reacts specifically with dynein of frog, hamster (CHO cell line), chicken, rat, marsupial (rat kangaroo, PtK1 cell line), Indian muntjac (Muntiacus muntjac vaginalis) and human (HeLa,MCF-7).
Monoclonal anti-Dynein (Intermediate Chain) (mouse IgM isotype) is derived from the 70.1 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cytoplasmic dynein purified from chick brain. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (approximately 450 kDa), three intermediate chains (70- 125 kDa), and at least four light chains (15-25 kDa). Dynein intermediate chain (ICs) is a component of dynein multisubunit. Dynein intermediate chain interacts with dynactin through its N-terminal region.

Imunogênio

cytoplasmic dynein from chicken embryo brain

Aplicação

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse has been used in:
  • perturbation assays
  • western blotting
  • immunofluorescence microscopy
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunocytochemistry
  • immunopurification

Ações bioquímicas/fisiológicas

Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Mechanically distinct microtubule arrays determine the length and force response of the meiotic spindle
Takagi J, et al.
Developmental Cell, 49(2), 267-278 (2019)
Co-silencing of human Bub3 and dynein highlights an antagonistic relationship in regulating kinetochore-microtubule attachments
Silva P, et al.
Febs Letters, 589(23), 3588-3594 (2015)
Marzia Pesaresi et al.
Biology of sex differences, 9(1), 6-6 (2018-01-21)
Disruption of axonal transport plays a pivotal role in diabetic neuropathy. A sex-dimorphism exists in the incidence and symptomatology of diabetic neuropathy; however, no studies so far have addressed sex differences in axonal motor proteins expression in early diabetes as
Firli R P Dewi et al.
Oncotarget, 9(17), 13337-13352 (2018-03-24)
Glycogen synthase kinase (GSK) 3β, which mediates fundamental cellular signaling pathways, has emerged as a potential therapeutic target for many types of cancer including colorectal cancer (CRC). During mitosis, GSK3β localizes in mitotic spindles and centrosomes, however its function is
Nucleoporin translocated promoter region (Tpr) associates with dynein complex, preventing chromosome lagging formation during mitosis
Nakano H, et al.
The Journal of Biological Chemistry, 285(14), 10841-10849 (2010)

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