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D4551

Sigma-Aldrich

N,N-Dimetilformamida

for molecular biology, ≥99%

Sinônimo(s):

DMF, NSC 5356

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About This Item

Fórmula linear:
HCON(CH3)2
Número CAS:
68-12-2
Peso molecular:
73.09
Beilstein:
605365
Número CE:
200-679-5
Número MDL:
MFCD00003284
Código UNSPSC:
12352111
ID de substância PubChem:
24893883
NACRES:
NA.52

grau

for molecular biology

Nível de qualidade

200

densidade de vapor

2.5 (vs air)

pressão de vapor

2.7 mmHg ( 20 °C)

Ensaio

≥99%

forma

liquid

temperatura de autoignição

833 °F

Lim. expl.

15.2 %

Impurezas

≤0.5% water
water

índice de refração

n20/D 1.430 (lit.)

pb

153 °C (lit.)

pf

−61 °C (lit.)

densidade

0.944 g/mL (lit.)

adequação

suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications.

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

[H]C(=O)N(C)C

InChI

1S/C3H7NO/c1-4(2)3-5/h3H,1-2H3

chave InChI

ZMXDDKWLCZADIW-UHFFFAOYSA-N

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Descrição geral

N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of hydrophobic organic compounds used in molecular biology applications.

Aplicação

N,N-Dimethylformamide was used to solubilize MTT crystals in cell viability assays. It was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme.
Solvente de muitos compostos orgânicos hidrofóbicos.

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

135.5 °F - closed cup

Ponto de fulgor (°C)

57.5 °C - closed cup

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Sigma-Aldrich

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Supelco

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Sigma-Aldrich

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Protocolos

cAMP Assays

cAMP measurements are obtained using an ELISA assay (Harlow and Lane 1988). Commercial radio-immunoassays, or ELISA kits, to assay cAMP can be purchased from various manufacturers.

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