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Key Documents

D1033

Sigma-Aldrich

Monoclonal Anti-Desmin antibody produced in mouse

clone DE-U-10, ascites fluid

Sinônimo(s):

Anti-CDCD3, Anti-CSM1, Anti-CSM2, Anti-LGMD1D, Anti-LGMD1E, Anti-LGMD2R

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

DE-U-10, monoclonal

peso molecular

antigen 50-55 kDa

contém

15 mM sodium azide

reatividade de espécies

rabbit, chicken, hamster, feline, lizard, sheep, rat, human, bovine, viper, mouse, goat

técnica(s)

indirect immunofluorescence: 1:40 using formalin-fixed, paraffin-embedded sections of human tissue
microarray: suitable
western blot: 1:200 using chicken gizzard extract/whole extract of mouse myoblast C2C12 cells

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

mouse ... Des(13346)
rat ... Des(64362)

Descrição geral

Desmin is the protein subunit of muscle-type intermediate filaments and is found predominantly in skeletal, cardiac, and smooth muscle.
Monoclonal Anti-Desmin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The desmin protein contains a head domain, four α-helical segments, three non-helical rod segments and a tail domain. It exists as dimer and tetramer assemblies. Desmin is is present in the periphery of Z-disk in smooth and striated muscles.

Especificidade

The antibody localizes desmin in cultured cells and tissue sections from several species and in tumors derived from muscle tissue (leiomyomas, rhabdomyosarcomas).

Imunogênio

desmin from pig stomach

Aplicação

Monoclonal Anti-Desmin antibody produced in mouse has been used in:
  • immunohistochemical staining
  • immunoblot analyses
  • immunocytochemistry
  • immunocytochemical localization of intermediate filaments

Monoclonal Anti-Desmin antibody produced in mouse is suitable for indirect immunofluorescence at a working dilution of 1:20 using formalin-fixed, paraffin-embedded sections of human tissue, microarray and western blotting. It may be used for immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs. It specifically stains desmin when used in immunoblotting assays. It may be used for labelling desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas). It was used for immunohistochemical staining of cardiac cells in a study to understand cardiac conduction through engineered tissue. It was used as a primary antibody for the detection of expression of desmin in cultured cells to study the effect of mutation in desmin gene.

Ações bioquímicas/fisiológicas

Desmin provides mechanical strength to muscle tissues. Mutations in desmin gene contributes to imbalance in skeletal proteins, resulting in desminopathies. Mutations in this gene damages the stability of intermediary filaments in cardiomyocytes.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Visite a Biblioteca de Documentos

K Rosenvold et al.
Journal of animal science, 88(5), 1830-1841 (2010-02-02)
The current pork slaughter process is primarily optimized to reduce cooler shrink and the incidence of PSE pork. Elimination of the halothane gene and improved preslaughter handling have decreased the incidence of PSE pork and improved the water-holding capacity of
Andreas Brodehl et al.
Circulation. Cardiovascular genetics, 6(6), 615-623 (2013-11-10)
The intermediate filament protein desmin is encoded by the gene DES and contributes to the mechanical stabilization of the striated muscle sarcomere and cell contacts within the cardiac intercalated disk. DES mutations cause severe skeletal and cardiac muscle diseases with
Functional characterization of the novel DES mutation p. L136P associated with dilated cardiomyopathy reveals a dominant filament assembly defect
Brodehl A, et al.
Journal of Molecular and Cellular Cardiology, 91, 207-214 (2016)
Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration
Park S, et al.
International Journal of Molecular Medicine, 37(5), 1209-1220 (2016)
Cardiac conduction through engineered tissue
Choi YH, et al.
The American Journal of Pathology, 169(1), 72-85 (2006)

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