CRISPR20V

Lenti CRISPR Universal Non-Target Control#3 Transduction Particles (LV04 vector)

• Código UNSPSC: 41106609
• NACRES: NA.51

Propriedades

• Formulário: liquid
• Nível de qualidade: 200
• embalagem: vial of 200 μL
• concentração: (1x10⁶ TU/mL (via p24 titering assay))
• aplicação(ões): CRISPR
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

The Lenti CRISPR Non-Target Control#3 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#3 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Control#3 uses a dual-component system consisting of U6-driven guide RNA (non-target#3) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

Aplicação

Functional Genomics/Screening/Target Validation

Outras notas

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

Informações legais

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable