SK-N-F1

94092304, human nerve (bone marrow metastasis), Epithelial-like

• Código UNSPSC: 41106514
• fonte biológica: human nerve (bone marrow metastasis)
• modo de crescimento: Adherent/aggregates in suspension
• cariótipo: Aneuploid
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Transferrin receptor
• doença(s) relevante(s): metastasis

Propriedades

• Nome do produto: SK-N-F1, 94092304
• fonte biológica: human nerve (bone marrow metastasis)
• modo de crescimento: Adherent/aggregates in suspension
• cariótipo: Aneuploid
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Transferrin receptor
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): metastasis
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human neuroblastoma

Descrição de linhagem celular

SK-N-F1 has been obtained from a bone marrow metastasis of a male patient with neuroblastoma. The poorly differentiated and slow growing cells secrete different neuroblastoma specific peptides and synthesise choline acetyltransferase. Reactivity of anti-leukaemia-associated and anti-foetal brain-associated monoclonal antibodies could be shown. Epithelial-like and aggregate-forming populations.

Aplicação

Carcinogenesis studies, cytotoxicity assays.

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,14
D16S539: 11,12
D5S818: 12,13
D7S820: 8,9
THO1: 6,9.3
TPOX: 8,9
vWA: 16,17

Meio de cultura

DMEM + 4mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/ml using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Clumped cells have tendency to float.

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