SK.N.SH Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012802, human nerve (bone marrow metastasis; Caucasian neuroblastoma), Epithelial

• Sinônimo(s): NSH Cells, SK-NSH Cells, SKNSH Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human nerve (bone marrow metastasis; Caucasian neuroblastoma)
• embalagem: tube of 5 μg 86012802-DNA-5UG; pkg of vial of cells 86012802-1VL
• modo de crescimento: Adherent
• cariótipo: 2n = 46, modal no. 47
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): metastasis
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian neuroblastoma

Descrição de linhagem celular

Established from a bone marrow metastasis from a 4-year-old caucasian female suffering with neuroblastoma. The cells have been used as a target cell in cell-mediated cytoxicity tests.

Aplicação

Human SK.N.SH cell line has been used in dark toxicity testing of a chloro-indium-phthalocyanine photosensitizer (In-Pc). It has also been used to study the potential mechanisms and domain requirements of the hHSJ1a (DnaJ heat shock protein family member B2) mediated reduction in superoxide dismutase 1 (SOD1) aggregation.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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