Neuro 2a Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89121404, mouse nerve, Neuronal/amoeboid-like

• Sinônimo(s): N2a Cells, Nb2a Cells, Neuro2a Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse nerve
• descrição: Mouse Albino neuroblastoma
• embalagem: tube of 5 μg 89121404-DNA-5UG; pkg of vial of cells 89121404-1VL
• modo de crescimento: Adherent
• cariótipo: 2n = 40
• morfologia: Neuronal/amoeboid-like
• produtos: Microtubular protein
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse Albino neuroblastoma

Descrição de linhagem celular

Derived from a spontaneous tumour in an albino strain A mouse. Cells produce microtubular protein which is believed to play a role in the contractile system giving axoplasmic flow in nerve cells.

Aplicação

Neuro 2a cell line has been used as a model to study the potential toxicological effects of copper oxide nanoparticles accumulation in the brain. It has also been used to determine the sensitivity of the cell line to saxitoxin.

Tumourigenicity studies, microtubular protein synthesis, vinblastine precipitation, GTP binding kinetics

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 4x10,000 cells cm²; 5% CO₂; 37°C. After resuscitation cells can take up to 6 days before a monolayer forms. These cells only adhere to the culture flask very lightly, care should be taken when subculturing. There is no need to wash the cells with PBS or trypsin, merely pour off half of the spent medium carefully, give the flask a gentle knock and resuspend the cells in fresh medium using a pipette to gently disperse any clumps of cells to finally reseed at 1:2. On arrival, growing cultures should be spun out and re-seeded in fresh media.

Outras notas

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